Introduction

Barley is the world's fourth-largest cereal crop and serves as a model organism for cellular biology, genetics, and barley genomics research. It has diverse applications in the animal feed, brewing, and distillation industries. We have developed the Barley Variation Database using high-throughput sequencing data generated during the next-generation sequencing era.

We conducted a comprehensive search and compilation of 536 whole exon sequencing samples, 1632 whole genome sequencing samples, 77 genotyping-by-sequencing samples, and 432 other barley sequencing datasets from public databases. Additionally, we incorporated 207 whole genome resequencing datasets completed by our research group to further enrich the database's content.

For each project, we offer functionalities such as variant information retrieval, gene haplotype retrieval, PCA analysis, evolutionary tree analysis, and population structure analysis. Based on our findings, we selected 40,529 sites with uniform genome coverage, high representativeness, and significant polymorphism, leading to the design of a new 40K barley breeding chip.

The Barley Variation Database (BarleyGVDB) also provides Blast alignment and JBrowse genome visualization tools, enabling researchers to efficiently utilize these valuable data resources for downstream analyses.